Immunohistochemical Localization of Transforming Growth Factor-/?1, -/?2, and -03 Latency-Associated Peptide in Human Cornea
نویسندگان
چکیده
Methods. Frozen sections obtained from eight human autopsy eyes were placed on gelatincoated slides. After blocking of nonspecific binding sites, the slides were incubated with rabbit polyclonal antibody to the latency-associated peptide (LAP) region of human TGF-01, TGF02, and TGF-/33 precursors, followed by the incubation with biotinylated swine anti-rabbit IgG. Subsequently, a streptavidin-labeled alkaline phosphatase technique was used.
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